10 international PhD fellowships in the European Doctoral Network BiocatCodeExpander for a training on innovative biotechnological applications of non-canonical amino acids for biocatalysis, synthetic biology, organic chemistry and computational biology.

PhD FELLOW # 1

STELA GALUSIC

WP1: TOOLS FOR NON-CANONICAL AMINO ACIDS INCORPORATION

‘High-throughput screening and selection methods for deep mutational scanning’

Name

Stela Galusic

Nickname

Organization
Graz University of Technology, Austria

Doctoral Supervisor
Prof. Robert Kourist 

Enrolment in Doctoral degree
Graz University of Technology (TUG)

Secondments
At Georgia Institute of Technology (Atlanta, United States of America) with Prof. Lynn Kamerlin
& moloX GmbH (Berlin, Germany) with Dr. Bernhard Loll

Bio / background

Tell us about your PhD

Tell us about your research interests – what do you expect from the consortium?

Tell us about your hobbies and motivations

Objectives

  • Development of high-throughput screening and selection methods for functional expression of non-canonical amino acids (NCAA)-containing enzymes
  • Develop whole-cell selection assays
  • Generation of gene site saturation mutagenesis libraries of NCAA-containing enzymes
  • Deep mutational scanning
  • Use machine learning for prediction of improved enzyme variants with extended substrate scope
  • Structure elucidation and biochemical characterization of improved variants

Fields related to the project

(1) Synthetic biology
(2) Computational biology
(3) Biocatalysis
(4) Structural biochemistry

    Marie Skłodowska-Curie Actions / Doctoral Networks

    This project has received funding from the European Union’s Horizon Europe research and innovation programme under the Marie Skłodowska-Curie grant agreement No 101072686.